Genomics, Behavior, Imaging Analysis are frequently used in our lab.
Below are some of the representative assays.
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1. Climbing Assay
Description: This is a behavioral assay used to measure fly climbing ability. It utilizes negative geotaxis behaviors of fruit flies and has been commonly used to monitor muscle functions. However, it should be noticed that both neuronal input and muscle strength contribute to the regulation of this behavior.
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2. Semi-Automated Optical Heartbeat Analysis (SOHA)
Description: This assay was first developed by Drs. Rolf Bodmer and Karen Ocorr. It has now been widely used in many labs to monitor the heart contractility in flies. For detail method, see links below.
Links: http://www.sohasoftware.com/ http://www.bodmerlab.org/ |
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3. Chromatin Immunoprecipitation (ChIP)
Description: ChIP is a very useful method used in the study of DNA-Protein interactions. Our lab primarily uses this method to investigate the genomic binding sites of transcription factors (TFs). See PDF file below for detailed protocol.
PDF download |
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4. Quantification of Drosophila Insulin-Like Peptides (DILPs) by ELISA
Description: This is a simple ELISA method that we developed to quantify circulating insulin-like peptides in fruit flies. See PDF file below for detailed protocol.
PDF download |